Transferrin Receptor (CD71)

Size

£659.00

SKU:: 169213
Purity:: Purified
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Human T-cells.
Clone:: 66IG10
Format:: Supplied as a liquid in PBS, 0.09% sodium azide.
Extra Details:: Transferrin receptor protein 1 (TfR1) also known as CD71 (Cluster of Differentiation 71) is a protein that in humans is encoded by the TFRC gene. CD71 plays a critical role in cell proliferation by controlling the supply of iron, which is essential for many metabolic pathways, through the binding and endocytosis of transferrin, the major iron-carrying protein. The expression of CD71 is regulated at the post-transcriptional level through the control of mRNA stability and is closely linked to intracellular iron levels. Nitric oxide can affect CD71 expression independently of intracellular iron concentration by activating proteins which then stabilize CD71 mRNA.Expression of CD71, or the transferrin receptor, is very low on resting leukocytes but is upregulated upon activation reflecting the iron dependence of activation. In other tissues, CD71 is expressed on most dividing cells and also on brain endothelium and alveolar macrophage. The ligand is the serum iron transport protein transferrin. CD71 antibodies are used as an indicator of proliferation in the typing of lymphomas and leukemias. Applications: Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Hybridoma: Sp2/0 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice