Neurofilament

Size

£659.00

SKU:: 169168
Purity:: Purified
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: human neurofilament preparation
Clone:: 2F11
Format:: Supplied as a liquid in PBS, 0.09% sodium azide.
Extra Details:: Like most other intermediate filament proteins (IFPs), the expression of the different neuronal IFPs is both tissue-specific and developmentally regulated. The neurofilament (NF) triplet proteins (70, 160, and 200kD) occur in both the central and peripheral nervous system and are normally restricted to neurons. The 70kD NF-protein can self-assemble into a filamentous structure, whereas the 160kD and 200kD NF-proteins require the presence of the 70kD NF-protein to co-assemble. All three NF proteins can be detected by immunohistochemical methods at day 9 or 10 after gestation in the mouse embryo.Although IFPs of the neurofilament type are normally restricted to neurons, there are reports on their expression in non-neuronal cells as well. For example, in heart conduction myocytes NF proteins are expressed together with desmin. In tumorpathology ganglioneuroblastomas and some of the other neuroblastomas are strongly positive with the neurofilament antisera. Also, some neuro-endocrine malignancies may show NF positivity. In cell cultures of neural tissues the neurofilament antibodies can monitor in vitro differentiation. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:100-1:1000 Immunohistochemistry (frozen, paraffin): 1:25-1:200. With avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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