Integrin alpha 5 (CD49e)

Size

£659.00

SKU:: 169103
Purity:: Purified
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Balb/c mice with human monocytes isolated by centrifugal elutriation.
Clone:: NKI-SAM-1
Format:: Supplied as a liquid in PBS, 0.09% sodium azide.
Extra Details:: ntegrins are transmembrane glycoproteins that belong to the family of adhesion molecules. They promote interactions between cells and their environment, being both other cells or the extra- cellular matrix. Integrins are a family of heterodimeric membrane glycoproteins consisting of non-covalently associated subunits, i.e. an a-subunit of 95kD that is conserved through the superfamily and a more variable b subunit of 150-170kD. More than 18 a and 8b subunits with numerous splice variant isoforms have been identified in mammals. The integrin a5 chain (160kD) (CD49e), undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light (25kD) and heavy (135kD) chains, which non-covalently associate with the integrin b1 subunit (CD29) (13kD), thus forming the heterodimer a5-1 very late antigen (VLA-5) complex. VLA-5 is a fibronectin receptor (FNr) that is expressed on thymocytes, T-cells, monocytes and platelets. It is also found on very early B-cells and activated B-cells. VLA-5 mediates binding of T- and B-cells to fibronectin, an extracellular matrix glycoprotein involved in cell adhesion and migration in wound healing, embryonic development and malignant transformation. The VLA-5 or FNr binds only fibronectin recognizing the Arg-Gly-Asp (RGD) sequence in the central region of the molecule. Applications: Suitable for use in Flow Cytometry, Immunoprecipitation and Immunohistochemistry. Other applications not tested. Recommended Dilution: Flow Cytometry: 0.5-1ug Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice