Ep-CAM (CD326)

Size

£659.00

SKU:: 169026
Purity:: Purified
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rat
Immunogen:: Glycoconjugates from Balb/C mouse derived medullary thymic epithelial cells.
Clone:: G8.8
Format:: Supplied as a liquid in PBS, 0.09% sodium azide.
Extra Details:: Ep-CAM is a 40kD glycoprotein and can be detected at the basolateral membrane of the majority of epithelial tissues, where it is intricately linked with the cadherin-catenin complex and hence the fundamental WNT pathway responsible for intracellular signalling and polarity. This antigen functions as a homotypic calcium-independent cell adhesion molecule. Of particular interest, Ep-CAM appears to be overexpressed by the majority of human epithelial carcinomas, including colorectal, breast, prostate, head and neck, and hepatic carcinomas. The antigen is being used as a target for immunotherapy of human carcinomas. Formation of Ep-CAM-mediated adhesions has a negative regulatory effect on adhesions mediated by classic cadherins, which may have strong effects on the differentiation and growth of epithelial cells. Ep-CAM overexpression was suggested to be associated with enhanced epithelial proliferation. Applications: Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:25-1:200 Western Blot: 1:100-1:1000 Immunohistochemistry (frozen): 1:25-1:200. With avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent. Optimal dilutions to be determined by the researcher. Hybridoma: X63-Ag8.653 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice