Collagen Type IV

Size

£659.00

SKU:: 168974
Purity:: Purified
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Human type IV collagen, isolated from human placenta.
Clone:: 1043
Format:: Supplied as a liquid in 0.09% sodium azide.
Extra Details:: Type IV collagen is an integral part of most, if not all basement membranes. The extent of expression of the basement membrane component type IV collagen may be correlated with the degree of differentiation of basement membrane producing neoplasms. Neoplastic cells of invasive carcinomas often lack a continuous basement membrane. In diagnostic histopathology antibodies to type IV collagen may help to distinguish between non-invasive (benign or in situ) and invasive lesions. Furthermore the antiserum can be of help in the differential diagnosis of bullous lesions in dermatopathology. Applications: Suitable for use in Flow Cytometry, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:100-1:1000 Immunohistochemistry (paraffin): 1:50-1:100 with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent. Pretreated with 1mg/ml pepsin in 0.01N HCl for 30 minutes at RT. Optimal dilutions to be determined by the researcher. Hybridoma: Sp2/0 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice