168972
Chromogranin A
Size
£524.00
- SKU:
- 168972
- Purity:
- Supernant
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Human pheochromocytoma
- Clone:
- LK2H10
- Format:
- Supplied as a liquid, 0.09% sodium azide.
- Extra Details:
- Neurons and neuroendocrine cells contain membrane-delimited pools of peptide hormones, biogenic amines, and neurotransmitters with a characteristic electron-dense appearance. These vesicles, which are present throughout the neuroendocrine system and in a variety of neurons, store and release chromogranins and secretogranins, a unique group of acidic, soluble secretory proteins. Chromogranin A, a protein of 439 amino acids which is encoded on chromosome 14, and an apparent molecular weight of 68kD, is present in neuroendocrine cells throughout the body, including the neuroendocrine cells of the large and small intestine, adrenal medulla and pancreatic islets. It is an excellent marker for carcinoid tumors, phenochromocytomas, paragangliomas, and other neuroendocrine tumors, including small cell lung carcinoma. Applications: Immunohistochemistry and Immunofluorescence. Other applications not tested. Recommended Dilution: Immunofluorescence: 1:10. With the ABC method the dilution can be 5-10x higher. Dilute immediately before use with PBS or TRIS-HCl pH 7,0. Optimal dilutions to be determined by the researcher. Hybridoma: NS1 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice