CD36

Size

£659.00

SKU:: 168966
Purity:: Purified
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Stimulated human leucocytes. Epitope: aa155-183
Clone:: 185-1G2
Format:: Supplied as a lyophilized powder in PBS, 0.09% sodium azide. Reconstitute with PBS, pH 7.2.
Extra Details:: The CD36 molecule is an 88kD transmembrane glycoprotein, which is a member of the class B scavenger receptor family of proteins. Alternative names for CD36 include fatty acid translocase (FAT), platelet glycoprotein 4, platelet glycoprotein IIIb and thrombospondin receptor. CD36 is a pattern recognition receptor that is expressed on a wide variety of cells including monocytes, macrophages, platelets, adipocytes, erythrocyte precursors, activated keratinocytes and some endothelial and epithelial cells. Studies suggest that CD36 has multiple cellular functions and binds to a diverse array of ligands including thrombospondin, modified low-density lipoproteins (LDL), anionic phospholipids and long chain fatty acids. As a scavenger receptor, CD36 plays a key role in mediating the uptake of oxidized LDL by macrophages, the formation of foam cells and the development of atherosclerosis. CD36 is also reported to function as negative regulator of angiogenesis, as a cell adhesion molecule involved in platelet adhesion and aggregation, and as a scavenger receptor in the clearance of apoptotic cells and photoreceptor rod outer segments. Furthermore, CD36 is reported to bind to Plasmodium falciparum infected erythrocytes and may play an important role in the pathogenesis of malaria and sickle cell disease. Applications: Suitable for use in Flow Cytometry, Immunohistochemistry and Immunocytochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry: frozen Flow Cytometry: 1:100-1:200 Optimal dilutions to be determined by the researcher. Hybridoma: NS1 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice