Actin, beta, cytoplasmic

Size

£659.00

SKU:: 168913
Purity:: Purified
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Peptide corresponding to the N-terminal nonapeptide of b-cytoplasmic actin with an acetylated N-terminus (Ac-DDDIAALVV-COOH), coupled to KLH.
Clone:: 4C2
Format:: Supplied as a liquid in PBS, 0.09% sodium azide.
Extra Details:: Among the six actin isoforms described in mammals, two are found in virtually all cells (b- and y-cytoplasmic), two are detected in smooth muscle cells (a- and y-smooth muscle) and two are present in striated muscles, one predominantly in skeletal (a-skeletal) and one in cardiac (a-cardiac) muscle cells. These actin isoforms differ slightly in their N-terminus, but the sequence of each of these actins is highly conserved in higher vertebrates. Alpha-smooth muscle actin is abundant in vascular and visceral smooth muscle cells. In addition, it has also been shown to appear in stress fibers of fibroblastic cells during pathological situations involving contractile phenomena such as wound healing and fibrocontractive diseases. Applications: Suitable for use in Western Blot, Immunocytochemistry. Other applications not tested. Hybridoma: NS0 myeloma cells with spleen cells from Balb/c mice. Recommended Dilution: Western Blot: 1:100-1:500 Immunohistochemistry (frozen, paraffin): 1:100-1:500. With avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice