168837-AF-ML650
MUC18 (CD146, Cell Surface Glycoprotein MUC18, Cell Surface Glycoprotein P1H12, Gicerin, MCAM, Melanoma Adhesion Molecule, Melanoma Associated Glycoprotein MUC18, Melanoma Cell Adhesion Molecule, Melanoma-associated Antigen A32, Mel-CAM, S-endo 1 Endothelial-associated Antigen, Sendo1) (BSA & Azide Free) (MaxLight 650)
Size
£876.00
- SKU:
- 168837-AF-ML650
- Application:
- FLISA
- Purity:
- Purified by Protein A/G affinity chromatography.
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant human MUC18 protein. Cellular Localization: Cell surface
- Clone:
- OJ79c
- Format:
- Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™650.
- Extra Details:
- MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000. The human Mel-CAM gene maps to chromosome 11q23 and encodes a trans-membrane glycoprotein, also designated MCAM, MUC 18 or CD146, that belongs to the immunoglobulin superfamily and functions as a Ca2+-independent cell adhesion molecule. Mel-CAM expression is restricted to advanced primary and metastatic melanomas and to cell lines of the neuroectodermal lineage, but not normal melanocytes. Mel-CAM is found on 80% of advanced primary human melanomas and correlates well with development of metastatic disease. Applications: Suitable for use in Immunofluorescence, Flow Cytometry, FLISA, Western Blot, Immunoprecipitation, Immunohistochemistry. Other applications not tested. Recommended Dilution: FLISA: For coating, order antibody without BSA Flow Cytometry: 0.5-1ug/million cells Immunofluorescence: 0.5-1.0ug/ml Immunoprecipitation: 1-2ug/500ug protein Western Blot: 0.5-1.0ug/ml Immunohistochemistry: Frozen: 0.5-1.0ug/ml for 30 min at RT Optimal dilutions to be determined by the researcher. Positive Control: A-375, HUV-EC or HeLa Cells. Tonsil or Melanoma. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice