168837-AF-BIOTIN

MUC18 (CD146, Cell Surface Glycoprotein MUC18, Cell Surface Glycoprotein P1H12, Gicerin, MCAM, Melanoma Adhesion Molecule, Melanoma Associated Glycoprotein MUC18, Melanoma Cell Adhesion Molecule, Melanoma-associated Antigen A32, Mel-CAM, S-endo 1 Endothelial-associated Antigen, Sendo1) (Biotin)

Size

£876.00

SKU:: 168837-AF-BIOTIN
Purity:: Purified by Protein A/G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant human MUC18 protein. Cellular Localization: Cell surface
Clone:: OJ79c
Format:: Supplied as a liquid in 10mM PBS. No preservative added. BSA-Free. Labeled with Biotin.
Extra Details:: The human Mel-CAM gene maps to chromosome 11q23 and encodes a trans-membrane glycoprotein, also designated MCAM, MUC 18 or CD146, that belongs to the immunoglobulin superfamily and functions as a Ca2+-independent cell adhesion molecule. Mel-CAM expression is restricted to advanced primary and metastatic melanomas and to cell lines of the neuroectodermal lineage, but not normal melanocytes. Mel-CAM is found on 80% of advanced primary human melanomas and correlates well with development of metastatic disease. Applications: Suitable for use in Immunofluorescence, Flow Cytometry, ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry. Other applications not tested. Recommended Dilution: ELISA: For coating, order antibody without BSA Flow Cytometry: 0.5-1ug/million cells Immunofluorescence: 0.5-1.0ug/ml Immunoprecipitation: 1-2ug/500ug protein Western Blot: 0.5-1.0ug/ml Immunohistochemistry: Frozen: 0.5-1.0ug/ml for 30 min at RT Optimal dilutions to be determined by the researcher. Positive Control: A-375, HUV-EC or HeLa Cells. Tonsil or Melanoma. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.

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