168835-AF

MUC1 (EMA, CD227, Breast carcinoma-associated antigen DF3, CA15-3, Carcinoma-associated mucin Episialin, Epithelial Membrane Antigen, H23AG, KL-6, MAM6, MUC-1, MUC-1/SEC, MUC-1/X, MUC1-alpha, MUC1-beta, MUC1-CT, MUC1-NT, MUC1/ZD, Mucin 1 cell surface associated, Mucin-1 subunit beta, Peanut-reactive urinary mucin, PEM, PEMT, Polymorphic epithelial mucin, PUM, Tumor-associated epithelial membrane antigen, Tumor-associated mucin) (BSA & Azide Free)

Size

£646.00

SKU:: 168835-AF
Purity:: Purified by Protein A/G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Delipidated extract of human milk fat globule membranes. Cellular Localization: Apical cell membrane, cell membrane, cytoplasm, nucleus, secreted.
Clone:: E29
Format:: Supplied as a liquid in PBS. Also available with BSA and azide, see 168835.
Extra Details:: In Western Blot, it recognizes proteins in MW range of 265-400kD , identified as different glycoforms of EMA. This protein is proteolytically cleaved into alpha and beta subunits that form a heterodimeric complex, the N-terminal alpha subunit and the C-terminal beta subunit. The alpha subunit has cell adhesive properties. It can act both as an adhesion and an anti-adhesion protein. EMA may provide a protective layer on epithelial cells against bacterial and enzyme attack. The beta subunit contains a C-terminal domain, which is involved in cell signaling, through phosphorylations and protein-protein interactions. In immunohistochemical assays, it superbly stains routine formalin/paraffin carcinomas. Antibody to EMA is useful as a pan-epithelial marker for detecting early metastatic loci of carcinoma in bone marrow or liver. Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 2-4ug/ml Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris, 1mM EDTA, pH 9.0 for 45 minutes at 95ºC followed by cooling at RT for 20 minutes. Optimal dilutions to be determined by the researcher. Positive Control: Colon, endpmetrisl carcinoma, MCF-7 or MDA-231 cells, breast, ovarian Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice