Mitochondria

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£422.00

SKU:: 168830
Purity:: Purified by Protein A/G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Semi-purified mitochondrial preparation. Cellular Localization: Mitochondria in cytoplasm
Clone:: 113-1
Format:: Supplied as a liquid in 10mM PBS, 0.05% BSA, 0.05% sodium azide. Also available without BSA and azide, see 168830-AF.
Extra Details:: MAb 113-1 recognizes a 60kDa antigen associated with the mitochondria in human cells. It can be used to stain mitochondria in cell or tissue preparations and can be used as a mitochondrial marker in subcellular fractions. It produces a spaghetti-like pattern in normal and malignant cells. This antibody is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells, including neurons and embryonic stem cells. Abundant granular eosinophilic cytoplasm is a common feature of renal oncocytoma, chromophobe renal cell carcinoma, eosinophilic variant of papillary renal cell carcinoma, and the granular variant of clear cell renal cell carcinoma (RCC). The chief reason for their distinction from one another is the difference in their biologic behavior. However, precise characterization may be difficult in some cases because of overlapping morphologic features. Immunostaining pattern with anti-mitochondrial MAb 113-1 has been reported as a useful discriminatory adjunct in the complex differential diagnosis of granular renal cell tumors. Moreover, Salivary gland tumors usually show great variability both in their morphopathology as well as their clinical behavior. One study highlights the usefulness of 113-1 MAb to facilitate the classification of salivary tumors, an aspect that may sometimes have not only diagnostic implications, but also prognostic. Applications: Suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunofluorescence: 0.5-1ug/ml Western Blot: 0.25-0.5ug/ml Immunoprecipitation: 0.5-1ug/500ug protein lysate Immunohistochemistry: Frozen & Formalin-fixed: 0.5-1ug/ml for 30 minutes at RT (Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 1mM EDTA Buffer, pH 8.5-9.5, for 10-20 min followed by cooling at RT for 20 minutes Optimal dilutions to be determined by the researcher. Positive Control: HeLa or HepG2 cells. Hepatic carcinoma. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice