168828-AF-BIOTIN

Melanoma Marker (MART-1 + Tyrosinase + gp100, Melanoma antigen recognized by T-cells 1 (MART-1), MLAN-A, TYR, PMEL17) (Biotin)

Size

£876.00

SKU:: 168828-AF-BIOTIN
Application:: IHC-Fr
Purity:: Purified by Protein A/G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant hMART-1 protein (A103); Recombinant tyrosinase protein (T311); Extract of pigmented melanoma metastases from lymph nodes (HMB45). Cellular Localization: Cytoplasmic
Clone:: A103, T311, HMB45
Format:: Supplied as a liquid in 10mM PBS. No preservative added. BSA-Free. Labeled with Biotin.
Extra Details:: Recognizes three melanoma-specific proteins, which include MART-1, Tyrosinase and gp100. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Tyrosinase is one of the targets for cytotoxic T-cell recognition in melanoma patients. The function of gp100 is not known but it is reported to be a useful marker for melanocytes and melanomas. This cocktail of three markers is designed for extremely sensitive labeling of formalin-fixed, paraffin-embedded melanomas and other tumors showing melanocytic differentiation. Applications: Suitable for use in Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry (Formalin-fixed): Requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes Optimal dilutions to be determined by the researcher. Positive Control: SK-MEL-13 and SK-MEL-19 Melanoma cell lines; Melanomas Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.

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