168814-AF-PE
Kappa Light Chain (HCAK1, Ig Kappa Chain C Region, IGKC, Immunoglobulin KM) (PE)
Size
£876.00
- SKU:
- 168814-AF-PE
- Purity:
- Purified by Protein A/G affinity chromatography.
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Human IgG-kappa myeloma protein. Cellular Localization: Cell membrane, secreted
- Clone:
- TB28-2
- Format:
- Supplied as a liquid in 10mM PBS. No preservative added. BSA-Free. Labeled with R-Phycoerythrin (PE).
- Extra Details:
- This MAb is specific to kappa light chain of immunoglobulin and shows no cross-reaction with lambda light chain or any of the five heavy chains. In mammals, the two light chains in an antibody are always identical, with only one type of light chain, kappa or lambda. The ratio of Kappa to Lambda is 70:30. However, with the occurrence of multiple myeloma or other B-cell malignancies this ratio is disturbed. Antibody to the kappa light chain is reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is malignant. Applications: Suitable for use in FLISA, Flow Cytometry and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Immunohistochemistry (Frozen/Formalin): Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 minutes at 95°C followed by cooling at RT for 20 minutes. Optimal dilutions to be determined by the researcher. Positive Control: 293T, Raji or hPBL cells. Tonsil or Spleen Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice