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168785-AF

HCG-alpha (CG-alpha, CGA, Chorionic Gonadotrophin Alpha, Follicle Stimulating Hormone Alpha, Follitropin Alpha, FSH-alpha, FSHA, GPH Alpha, GPHA1, LHA, LH-alpha, Luteinizing Hormone Alpha, Lutropin Alpha, Thyroid Stimulating Hormone Alpha, Thyrotropin Alpha, TSHA) (BSA & Azide Free)

Size

£646.00

SKU:
168785-AF
Purity:
Purified by Protein A/G affinity chromatography.
Storage Conditions:
-20°C
Supplier:
United States Biological
Host:
Mouse
Immunogen:
Recombinant hCG alpha protein. Cellular Localization: Cytoplasmic, secreted
Clone:
HCGa/53
Format:
Supplied as a liquid in 10mM PBS. Also available with BSA and azide, see 168785.
Extra Details:
This MAb reacts with a protein of ~13kD, identified as alpha sub-unit of HCG. HCG is a glycoprotein, which is secreted in large quantities by normal trophoblasts. It is present only in trace amounts in non-pregnant urine and sera but rises sharply during pregnancy. HCG is composed of two non-identical, non-covalently linked polypeptide chains designated as the a and b subunits. The a subunit is identical to that of thyroid stimulating hormone (TSH), follicle stimulating hormone (FSH), and luteinizing hormone (LH). Applications:  Suitable for use in ELISA, Western Blot, Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 0.5-1ug/ml Immunohistochemistry: formalin-fixed: 0.5-1ug/ml for 30 minutes at RT (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes) Optimal dilution for a specific application should be determined Positive Control: Placenta Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Shipping Conditions:
Blue Ice