168784-AF-HRP

HCC-4 (C-C motif chemokine 16 CCL16, Chemokine CC-4, CKb12, HCC-4, IL-10-inducible chemokine (ILINCK), LCC-1, Liver-expressed chemokine (Chemokine LEC), Lymphocyte and monocyte chemoattractant (LMC), Monotactin-1 (MTN-1), New CC chemokine 4 (NCC-4), SCYL4, Small-inducible cytokine A16, SCYA16) (HRP)

Size

£876.00

SKU:: 168784-AF-HRP
Purity:: Purified by Protein A/G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant human HCC-4 protein. Cellular Localization: Secreted (extracellular)
Clone:: LEC67
Format:: Supplied as a liquid in 10mM PBS. No preservative added. BSA-Free. Labeled with horseradish peroxidase (HRP).
Extra Details:: Shows chemotactic activity for lymphocytes and monocytes but not neutrophils. Also shows potent myelo-suppressive activity, suppresses proliferation of myeloid progenitor cells. Recombinant SCYA16 shows chemotactic activity for monocytes and THP-1 monocytes, but not for resting lymphocytes and neutrophils. Induces a calcium flux in THP-1 cells that were desensitized by prior expression to RANTES. Mainly expressed in liver, also found in spleen and thymus. Highly expressed in LPS- and IFN-gamma-activated monocytes, weakly in some lymphocytes, including natural killer cells, gamma-delta T-cells, and some T-cell clones. The ability of HCC-4 to markedly improve recognition of poorly immunogenic cells suggests that it could be an effective component of antitumor vaccines. Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Positive Control: Liver, spleen and thymus Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.

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