Golgi Complex

Size

£422.00

SKU:: 168781
Purity:: Purified by Protein A/G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: SU-DHL-1 large cell lymphoma cells. Cellular Localization: Golgi complex in cytoplasm
Clone:: 371-4
Format:: Supplied as a liquid in 10mM PBS, 0.05% BSA, 0.05% sodium azide. Also available without BSA and azide, see 168781-AF.
Extra Details:: MAb 371-4 recognizes Golgi complex in human cells. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. MAb 371-4 recognizes an antigen associated with the Golgi complex in human cells only. It can be used to stain the Golgi complex in cell or tissue preparations and can be used as a Golgi marker in subcellular fractions. It produces a diffuse staining pattern of the Golgi zone in normal and malignant cells and may be used to stain Golgi complex of cells in frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations. MAb 371-4 is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells. Applications: Suitable for use in Immunofluorescence, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: Immunofluorescence: 0.5-1ug/ml; Immunocytochemistry (Acetone or paraformaldehyde fixed): 0.5-1ug/ml for 30 min Immunohistochemistry (Frozen): 0.5-1ug/ml for 30 minutes at RT Optimal dilutions to be determined by the researcher. Positive Control: Tonsil or lymph node Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice