Ep-CAM (CD326, Adenocarcinoma-associated Antigen, Cell Surface Glycoprotein Trop-1, EGP2, EGP314, EGP40, Epithelial Cell Adhesion Molecule, Epithelial Glycoprotein 314, ESA, KSA, TACD1, TACSTD1, TROP1, Tumor-associated Calcium Signal Transducer 1, ECS-1, Epidermal Surface Antigen 1, ESA1, FLOT2, Flotillin-2, M17S1 (Membrane Component, Chromosome 17, Surface Marker-1), REG-1, Reggie-1, Reggie-2)

Size

£422.00

SKU:: 168769
Purity:: Purified by Protein A/G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: MCF-7 human breast cancer cells. Cellular Localization: Cell surface
Clone:: 323/A3
Format:: Supplied as a liquid in 10mM PBS, 0.05% BSA, 0.05% sodium azide. Also available without BSA and azide, see 168769-AF.
Extra Details:: EGP40 is a 40-43kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma. Applications: Suitable for use in Immunofluorescence, Flow Cytometry, ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry. Other applications not tested. Recommended Dilution: Flow Cytometry: 0.5-1ug/million cells Immunofluorescence: 1-2ug/ml Immunoprecipitation: 1-2ug/500ug protein Western Blot: 0.25-0.5ug/ml Immunohistochemistry: Frozen & formalin-fixed: 0.5-1.0ug/ml for 30 min at RT (Staining of formalin/paraffin tissues REQUIRES digestion of tissue sections with pepsin at 1mg/ml Tris-HCl, pH 2.0 for 15 min at RT or 10 min at 37C Optimal dilutions to be determined by the researcher. Positive Control: HT29 cells or breast tumor Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice