168766-AF-BIOTIN
EGFR (Erbb1, ERBB1, Errp, HER1, mENA, PIG61, Proto-oncogene c-ErbB-1, Receptor Tyrosine Protein Kinase, ErbB1, Urogastrone, wa2, Wa5) (Biotin)
Size
£876.00
- SKU:
- 168766-AF-BIOTIN
- Purity:
- Purified by Protein A/G affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Purified EGFR from A431 cells.. Cellular Localization: Cell surface
- Clone:
- H9B4
- Format:
- Supplied as a liquid in 10mM PBS. No preservative added. BSA-Free. Labeled with Biotin.
- Extra Details:
- This MAb reacts with a cytoplasmic domain of EGFR. EGFR is a type I receptor tyrosine kinase with sequence homology to erbB-1, -2, -3 -4 or HER-1, -2, -3 -4. It binds to Epidermal Growth Factor (EGF), Transforming Growth Factor-a (TGF-a), Heparin-binding EGF (HB-EGF), amphiregulin, betacellulin & epiregulin. Applications: Suitable for use in ELISA, Immunoprecipitation, Western Blot. Other applications not tested. Recommended Dilution: Immunoprecipitation: 1-2ug/500ug protein lysate Western Blot: 0.5-1.0ug/ml for 2 hours at RT Optimal dilutions to be determined by the researcher. Positive Control: A431 cells, Breast or Bladder cancer Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice