168766-AF-APC

EGFR (Erbb1, ERBB1, Errp, HER1, mENA, PIG61, Proto-oncogene c-ErbB-1, Receptor Tyrosine Protein Kinase, ErbB1, Urogastrone, wa2, Wa5) (APC)

Size

£876.00

SKU:: 168766-AF-APC
Purity:: Purified by Protein A/G affinity chromatography.
Storage Conditions:: 4°C Do Not Freeze
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Purified EGFR from A431 cells.. Cellular Localization: Cell surface
Clone:: H9B4
Format:: Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).
Extra Details:: This MAb reacts with a cytoplasmic domain of EGFR. EGFR is a type I receptor tyrosine kinase with sequence homology to erbB-1, -2, -3 -4 or HER-1, -2, -3 -4. It binds to Epidermal Growth Factor (EGF), Transforming Growth Factor-a (TGF-a), Heparin-binding EGF (HB-EGF), amphiregulin, betacellulin & epiregulin. Applications: Suitable for use in ELISA, Immunoprecipitation, Western Blot. Other applications not tested. Recommended Dilution: Immunoprecipitation: 1-2ug/500ug protein lysate Western Blot: 0.5-1.0ug/ml for 2 hours at RT Optimal dilutions to be determined by the researcher. Positive Control: A431 cells, Breast or Bladder cancer Storage and Stability: May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. APC is light sensitive. Note: Applications are based on unconjugated antibody.