168765-AF
EGFR (Erbb1, ERBB1, Errp, HER1, mENA, PIG61, Proto-oncogene c-ErbB-1, Receptor Tyrosine Protein Kinase, ErbB1, Urogastrone, wa2, Wa5) (BSA & Azide Free)
Size
£646.00
- SKU:
- 168765-AF
- Application:
- IP
- Purity:
- Purified by Protein A/G affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Microsomes from A431 cells. Cellular Localization: Cell surface
- Clone:
- B1D8
- Format:
- Supplied as a liquid in 10mM PBS. Also available with BSA and azide, see 168765.
- Extra Details:
- This antibody reacts with the extracellular domain of EGFR and blocks the EGF/TGFa−induced activation. It also blocks tumor growth in vivo. It is excellent for purification of EGFR. EGFR is type I receptor tyrosine kinase with sequence homology to erbB-1, -2, -3 -4 or HER-1, -2, -3 -4. It binds to Epidermal Growth Factor (EGF), Transforming Growth Factor-a (TGF-a), Heparin-binding EGF (HB-EGF), amphiregulin, betacellulin and epiregulin. Applications: Suitable for use in Flow Cytometry, Immunoprecipitation, Affinity Purification, Inhibits Proliferation, Arrests Tumor Growth in vivo. Other applications not tested. Recommended Dilution: Immunoprecipitation: 1-2ug/500ug protein lysate Flow Cytometry: 0.5-1ug/million cells Optimal dilutions to be determined by the researcher. Positive Control: A431 cells, Breast or Bladder cancer Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice