168764-AF

EBV Early Antigens (EBNA1, EBV nuclear antigen 1, Epstein Barr nuclear antigen 1, Human Herpesvirus 4 (HHV4)) (BSA & Azide Free)

Size

£646.00

SKU:: 168764-AF
Application:: IF
Purity:: Purified by Protein A/G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Affinity purified early antigen polypeptides from induced Raji cells precipitated with African Burkitt’s lymphoma sera. Cellular Localization: Nuclear
Clone:: 1108-1
Format:: Supplied as a liquid in 10mM PBS. Also available with BSA and azide, see 168764.
Extra Details:: Epstein-Barr virus (EBV), also designated human herpesvirus 4 (HHV-4), is a member of the herpesvirus family and is one of the most common human viruses. EBV infects B cells and, though often asymptomatic, it can cause infectious mononucleosis, a disease characterized by fatigue, fever, sore throat and muscle soreness. The EBV-induced early antigens (Ea) are among several antigen complexes that have been identified in EBV-infected cells. The Ea complex is composed of diffuse (Ea-D) and restricted (Ea-R) components. The activity of Ea-D is suppressed during latent infection. BMRF1, the gene that encodes for Ea-D, is closely associated with the gene encoding for EBV DNA polymerase, and Ea-D is essential for the activity of this polymerase. Ea-D forms a complex with EBV DNase and, together, they may play a role in viral replication. Applications: Suitable for use in Immunofluorescence Other applications not tested. Recommended Dilution: Immunofluorescence (cells): 0.5-1ug/ml Optimal dilutions to be determined by the researcher. Positive Control: EBV-EBV-infected cells Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice