168764-AF-BIOTIN

EBV Early Antigens (EBNA1, EBV nuclear antigen 1, Epstein Barr nuclear antigen 1, Human Herpesvirus 4 (HHV4)) (Biotin)

Size

£876.00

SKU:: 168764-AF-BIOTIN
Application:: IF
Purity:: Purified by Protein A/G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Affinity purified early antigen polypeptides from induced Raji cells precipitated with African Burkitt’s lymphoma sera. Cellular Localization: Nuclear
Clone:: 1108-1
Format:: Supplied as a liquid in 10mM PBS. No preservative added. BSA-Free. Labeled with Biotin.
Extra Details:: Epstein-Barr virus (EBV), also designated human herpesvirus 4 (HHV-4), is a member of the herpesvirus family and is one of the most common human viruses. EBV infects B cells and, though often asymptomatic, it can cause infectious mononucleosis, a disease characterized by fatigue, fever, sore throat and muscle soreness. The EBV-induced early antigens (Ea) are among several antigen complexes that have been identified in EBV-infected cells. The Ea complex is composed of diffuse (Ea-D) and restricted (Ea-R) components. The activity of Ea-D is suppressed during latent infection. BMRF1, the gene that encodes for Ea-D, is closely associated with the gene encoding for EBV DNA polymerase, and Ea-D is essential for the activity of this polymerase. Ea-D forms a complex with EBV DNase and, together, they may play a role in viral replication. Applications: Suitable for use in Immunofluorescence Other applications not tested. Recommended Dilution: Immunofluorescence: 0.5-1ug/ml Optimal dilutions to be determined by the researcher. Positive Control: EBV-EBV-infected cells or tissues Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.

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