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168760-AF-HRP

Cytomegalovirus p65 (CMV p65) (HRP)

Size

£876.00

SKU:
168760-AF-HRP
Purity:
Purified by Protein A/G affinity chromatography.
Storage Conditions:
-20°C
Supplier:
United States Biological
Host:
Mouse
Immunogen:
Recombinant CMV-p65 protein. Cellular Localization: Nuclear
Clone:
CMV101
Format:
Supplied as a liquid in 10mM PBS. No preservative added. BSA-Free. Labeled with horseradish peroxidase (HRP).
Extra Details:
Ten to forty percent of the patients with acquired immunodeficiency syndrome (AIDS) develop cytomegalovirus (CMV) infections. In some patients with AIDS, CMV is detected in the bronchoalveolar lavage fluid (BALF), urine, and other specimens, even when there are no symptoms of CMV disease. An indicator of active CMV infection is needed to facilitate the diagnosis of CMV disease in patients with AIDS or HIV infection. CMV p65 antigen was detected in the leukocytes of both the peripheral blood and BALF during the early phase of CMV disease. Applications:  Suitable for use in ELISA, Immunocytochemistry. Other applications not tested. Recommended Dilution: ELISA: For coating, order Ab without BSA Immunocytochemistry: Acetone or paraformaldehyde fixed: 0.5-1ug/ml for 30 min Optimal dilutions to be determined by the researcher. Positive Control: CMV-infected cells or tissues. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
Shipping Conditions:
Blue Ice