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168759-AF-ML490

Cytomegalovirus p65 (CMV p65) (BSA & Azide Free) (MaxLight 490)

Size

£876.00

SKU:
168759-AF-ML490
Application:
FLISA
Purity:
Purified by Protein A/G affinity chromatography.
Storage Conditions:
4°C Do Not Freeze
Supplier:
United States Biological
Host:
Mouse
Immunogen:
Recombinant CMV-p65 protein. Cellular Localization: Nuclear
Clone:
CMV100
Format:
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™490.
Extra Details:
MaxLight™490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (491nm); Emission (515nm); Extinction Coefficient 73,000. Ten to forty percent of the patients with acquired immunodeficiency syndrome (AIDS) develop cytomegalovirus (CMV) infections. In some patients with AIDS, CMV is detected in the bronchoalveolar lavage fluid (BALF), urine, and other specimens, even when there are no symptoms of CMV disease. An indicator of active CMV infection is needed to facilitate the diagnosis of CMV disease in patients with AIDS or HIV infection. CMV p65 antigen was detected in the leukocytes of both the peripheral blood and BALF during the early phase of CMV disease. Applications:  Suitable for use in Immunofluorescence, FLISA, Immunocytochemistry. Other applications not tested. Recommended Dilution: FLISA: For coating, order Ab without BSA Immunocytochemistry: Acetone or paraformaldehyde fixed: 0.5-1ug/ml for 30 min Immunofluorescence: 0.5-1ug/ml  Optimal dilutions to be determined by the researcher. Positive Control: CMV-infected cells or tissues. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.  Note: Applications are based on unconjugated antibody.
Shipping Conditions:
Blue Ice