168756-AF

Cytokeratin 8/18 (CARD2, CK8, CYK8, CYKER, Cytokeratin Endo A, DreK8, EndoA, K2C8, K8, Keratin 8, Krt 2.8, KRT8, Type-II Keratin Kb8. Cell Proliferation-inducing Gene 46 Protein, CK18, CYK18 Cytokeratin Endo B, K18, Keratin-18, Kerd, KRT18) (BSA & Azide Free)

Size

£646.00

SKU:: 168756-AF
Purity:: Purified by Protein A/G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Keratin preparation from a human carcinoma (K8.8); PMC-42 human breast carcinoma cells (DC10). Cellular Localization: Cytoplasmic.
Clone:: K8.8, DC10
Format:: Supplied as a liquid in 10mM PBS. Also available with BSA and azide, see 168756.
Extra Details:: Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18). This MAb cocktail recognizes all simple epithelia including glandular epithelium, for example thyroid, female breast, gastrointestinal tract, respiratory tract, and urogenital tract including transitional epithelium. All adenocarcinomas and most squamous carcinomas are positive but keratinizing squamous carcinomas are usually negative. Immunohistochemical staining with this MAb is indistinguishable from that obtained with monoclonal antibody 5D3. Applications: Suitable for use in Immunofluorescence, Flow Cytometry, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Immunohistochemistry (Formalin): Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0,for 45 minutes at 95°C, followed by cooling at RT for 20 minutes. Optimal dilutions to be determined by the researcher. Positive Control: MCF-7 or A431 cells. Skin, Colon, lung or breast carcinoma Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice