168744-AF
Cyclin A (CCN1, CCNA, CCNA2, Cyclin A2) (BSA & Azide Free)
Size
£646.00
- SKU:
- 168744-AF
- Purity:
- Purified by Protein A/G affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Full length bovine Cyclin A protein. Cellular Localization: Predominantly nuclear.
- Clone:
- E67
- Format:
- Supplied as a liquid in 10mM PBS. Also available with BSA and azide, see 168744.
- Extra Details:
- Cyclins are regulatory subunits of the cyclin-dependent kinases (cdk’s) and they control transition at different specific phases of the cell cycle. The temporal expression of cyclins is tightly regulated and subsequently plays a critical role in controlling the enzymatic activity of cdk’s. These cyclin/cdk complexes are essential for passage through specific stages in the cell cycle. In mammalian somatic cells, cyclin A is required for S-phase and passage through G2-phase. The D and E type cyclins regulate the passage of G1, while cyclin B is a critical regulator of mitosis. Mutation or disruption of normal cyclin A expression causes cells to arrest in G2-phase. Applications: Suitable for use in Immunofluorescence, Flow Cytometry, ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Immunofluorescence: 1-2ug/ml Flow Cytometry: 0.5-1ug/10e6 cells Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Optimal dilutions to be determined by the researcher. Positive Control: K-562 and HeLa Cells. Human tonsil and lymph node Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice