168717-AF-BIOTIN

CD71 (Transferrin Receptor, Mtvr-1, p90, TFR1, TFRC transferrin receptor (p90 CD71), TRFR) (Biotin)

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£876.00

SKU:: 168717-AF-BIOTIN
Purity:: Purified by Protein A/G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: KG1 acute myeloid leukemia cell line. Cellular Localization: Cell surface
Clone:: DF1513
Format:: Supplied as a liquid in 10mM PBS. No preservative added. BSA-Free. Labeled with Biotin.
Extra Details:: It recognizes a ~90-95kDa protein which is identified as cell surface transferrin receptor (CD71), a disulfide-bonded homodimeric glycoprotein of 180-190kDa (Workshop IV). This MAb is highly specific to CD71 and shows no cross-reaction with other related proteins. Ligand for transferrin receptor is the serum iron transport protein, transferrin. This receptor is broadly distributed in carcinomas, sarcomas, leukemias, and lymphomas. CD71/Transferrin receptor has been reported to be associated with cell proliferation in both normal and neoplastic tissues and useful in predicting clinical behavior or response to therapy in a number of malignancies including breast cancer. Applications: Suitable for use in Immunofluorescence, ELISA, Flow Cytometry, Western Blot, Immunoprecipitation. Other applications not tested. Recommended Dilution: Flow Cytometry: 0.5-1ug/million cells Immunofluorescence: 1-2ug/ml Western Blot: 0.25-0.5ug/ml Immunohistochemistry: Frozen & formalin-fixed: 1-2ug/ml for 30 min at RT (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes Optimal dilutions to be determined by the researcher. Positive Control: CCRF-CEM, Jurkat or K-562. Human placenta or breast carcinoma. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.

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