168701-AF-BIOTIN

CD47 (Antigenic Surface Determinant Protein OA3, IAP, Integrin Associated Protein, Integrin Associated Signal Transducer, MER6, OA3, Protein MER6, Rh Related Antigen) (Biotin)

Size

£876.00

SKU:: 168701-AF-BIOTIN
Purity:: Purified by Protein A/G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Intact CD47 purified from placenta. Cellular Localization: Cell surface, cytoplasmic
Clone:: B6H12.2
Format:: Supplied as a liquid in 10mM PBS. No preservative added. BSA-Free. Labeled with Biotin.
Extra Details:: This antibody reacts with Ig domain of CD47 protein. It has been shown to inhibit polymorphonuclear neutrophil (PMN) transmigration across cell monolayers and matrix. CD47, originally named integrin-associated protein (IAP), is a 50kDa protein containing five membrane-spanning sequences and a short cytoplasmic tail. CD47 plays a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. It is important in memory formation and synaptic plasticity in the hippocampus. CD47 may play a role in membrane transport and/or integrin dependent signal transduction. Applications: Suitable for use in Immunofluorescence, Flow Cytometry, Western Blot, Immunoprecipitation, Immunohistochemistry, Functional Studies. Other applications not tested. Recommended Dilution: Functional Studies: Order Ab without Azide Flow Cytometry: 0.5-1ug/million cells Immunofluorescence: 1-2ug/ml Immunoprecipitation: 1-2ug/500ug protein Western Blot: 0.5-1ug/ml Immunohistochemistry: Frozen: 0.5-1ug/ml for 30 min at RT Optimal dilutions to be determined by the researcher. Positive Control: HUVEC, OVCA3 cells, Placenta, Brain and Ovarian Tumors Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.

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