CD31 (PECAM-1, EndoCAM, PECA1, Platelet Endothelial Cell Adhesion Molecule 1, GPIIA')

Size

£422.00

SKU:: 168685
Purity:: Purified by Protein A/G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Human recombinant CD31 protein (C31.3) and membrane preparation of a spleen from a patient with hairy cell leukemia (JC/70A). Cellular Localization: Cell surface and cytoplasm of endothelial cells
Clone:: C31.3, JC/70A
Format:: Supplied as a liquid in 10mM PBS, 0.05% BSA, 0.05% sodium azide.
Extra Details:: CD31 (PECAM-1) is a transmembrane glycoprotein member of the immunoglobulin supergene family of adhesion molecules. CD31 is expressed by stem cells of the hematopoietic system and is primarily used to identify and concentrate these cells for experimental studies as well as for bone marrow transplantation. Anti-CD31 has shown to be highly specific and sensitive for vascular endothelial cells. Staining of nonvascular tumors (excluding hematopoietic neoplasms) is rare. CD31 MAb reacts with normal, benign, and malignant endothelial cells which make up blood vessel lining. The level of CD31 expression can help to determine the degree of tumor angiogenesis, and a high level of CD31 expression may imply a rapidly growing tumor and potentially a predictor of tumor recurrence. Applications: Suitable for use in Immunofluorescence, Flow Cytometry, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 1-2ug/10e6 cells Western Blot: 1-2ug/ml Immunofluorescence: 1-2ug/ml Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10mM Tris with 1mM EDTA pH 9.0, for 10-20 minutess followed by coooling at RT for 20 minutes. Optimal dilutions to be determined by the researcher. Positive Control: Jurkat cells, Tonsil, Angiosarcoma Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice