CD28 (T-cell-specific surface glycoprotein CD28, Tp44) (PE)

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£646.00

SKU:: 168684
Application:: Flow Cytometry
Purity:: Purified by Protein A/G affinity chromatography.
Storage Conditions:: 4°C Do Not Freeze
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant human CD28 protein. Cellular Localization: Cell surface
Clone:: CB28
Format:: Supplied as a liquid in 10mM PBS, 0.05% BSA, 0.05% sodium azide. Labeled with R-Phycoerythrin (PE).
Extra Details:: Recognizes a glycoprotein of 44-88kDa, which is identified as CD28. It is the critical T-cell co-stimulatory receptor which provides to the cell the important second activation signal by binding CD80 and CD86 that are expressed by antigen presenting cells. Besides its co-stimulation role, CD28 functions in preventing T-cells from anergic hyporesponsive state or from undergoing premature apoptotic cell death. CD28 is also expressed on human fetal NK cells and some NK cell lines, whereas on murine NK cells the CD28 expression is much broader. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 2.5ul: 0.5ug/test/1e6 cells Optimal dilutions to be determined by the researcher. Positive Control: Jurkat cells or human lymph nodes and tonsils. Storage and Stability: May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Stable for 12 months after receipt at 4°C. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light.

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