CD20 (APY, ATOPY, B-lymphocyte antigen CD20, B-lymphocyte cell-surface antigen B1, Bp35, Fc epsilon receptor I beta chain, Fc Fragment of IgE high affinity I receptor for beta polypeptide, FCER1B, High affinity immunoglobulin epsilon receptor subunit beta, IgE Fc receptor subunit beta, IGEL, IGER, IGHER, LEU16, Leukocyte surface antigen Leu-16, Ly44, Membrane spanning 4 domains subfamily A member 2, Membrane-spanning 4-domains subfamily A member 1, MS4A1) (PE)

Size

£646.00

SKU:: 168679
Application:: Flow Cytometry
Purity:: Purified by Protein A/G affinity chromatography.
Storage Conditions:: 4°C Do Not Freeze
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Lymphoblastoid cell line Daudi. Cellular Localization: Predominantly cell surface with some cytoplasmic
Clone:: B9E9
Format:: Supplied as a liquid in 10mM PBS, 0.05% BSA, 0.05% sodium azide. Labeled with R-Phycoerythrin (PE).
Extra Details:: Recognizes a protein of 33-37kDa, identified as CD20 (Workshop V; Code CD20.12). B9E9 recognizes extracellular domain of CD20. The epitope is similar to or identical to that recognized by other CD20 antibodies including Leu-16 and B1. This MAb can be used for immunophenotyping of leukemia and malignant cells, B lymphocyte detection in peripheral blood, B cell localization in tissues and B lymphocyte purification by immunosorbent methods. CD20 is a non-Ig differentiation antigen of B-cells and its expression is restricted to normal and neoplastic B-cells, being absent from all other leukocytes and tissues. CD20 is expressed by pre B-cells and persists during all stages of B-cell maturation but is lost upon terminal differentiation into plasma cells. Protein passes through the membrane 4 times with both ends in cytoplasm and exposes one short and one longer loop to the external environment. CD20 is not glycosylated in resting B cells and its cytoplasmic domains are differentially phosphorylated upon activation. It acts as a calcium channel involved in B-cell activation and cell cycle progression. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 2.5ul: 0.5ug/test/1e6 cells Optimal dilutions to be determined by the researcher. Positive Control: Daudi, Raji, and U266, and human lymphocytes. In normal lymph nodes and tonsils, CD20 is predominantly detected on B cells in germinal centers. Storage and Stability: May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Stable for 12 months after receipt at 4°C. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light.

Shipping Conditions:: Blue Ice