168663-AF-ML550
CD11b (CD11 antigen-like family member B, CD11b/CD18, CD49d, Cell surface glycoprotein MAC-1 subunit alpha, Complement Component Receptor 3 Alpha, CR3 Alpha Chain (CR3A), Integrin alpha-M, Integrin beta 2 alpha subunit, ITGAM, Leukocyte adhesion receptor MO1, Ly-40, Mac-1a, MAC1, Mac1, alpha subunit, MAC1A, Macrophage antigen alpha polypeptide, MO1A, Neutrophil adherence receptor alpha M subunit) (BSA & Azide Free) (MaxLight 550)
Size
£876.00
- SKU:
- 168663-AF-ML550
- Application:
- FLISA
- Purity:
- Purified by Protein A/G affinity chromatography.
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant human CD11b protein. Cellular Localization: Cell surface
- Clone:
- C11b/660
- Format:
- Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™550.
- Extra Details:
- MaxLight™550 is a new Yellow-Green photostable dye conjugate comparable to Alexa Fluor™546, 555, DyLight™549 , Cy3™, TRITC and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (550nm); Emission (575nm); Extinction Coefficient 150,000. CD11b is a cell adhesion molecule that acts as a receptor for cell surface ligands such as intracellular adhesion molecules (ICAMs) or soluble ligands. Integrins are heterodimeric proteins that contain an a chain and b chain. Integrin åM combines with the Integrin ∫2 to form a leukocyte-specific integrin referred to as macrophage receptor 1 (Mac-1), or inactivated-C3b (iC3b) receptor 3 (CR3). Integrin åM/∫2 is important in the adherence of neutrophils and monocytes to stimulated endothelium, and also in the phagocytosis of complement coated particles. Applications: Suitable for use in Immunofluorescence, Flow Cytometry, FLISA, Immunoprecipitation, Immunohistochemistry, Functional Studies. Other applications not tested. Recommended Dilution: Flow Cytometry: 0.5-1ug/million cells Immunofluorescence: 0.5-1ug/ml Immunoprecipitation: 0.5-1ug/500ug protein lysate Immunohistochemistry: Frozen: 0.5-1.0ug/ml for 30 minutes at RT Optimal dilutions to be determined by the researcher. Positive Control: Human monocytes & granulocytes. Human lymph nodes and tonsils. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice