168650
Biotin (Vitamin B7, Vitamin H)
Size
£422.00
- SKU:
- 168650
- Purity:
- Purified by Protein A/G affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Biotinylated sheep immunoglobulin. Cellular Localization: Depends on the target
- Clone:
- Hyb-8
- Format:
- Supplied as a liquid in 10mM PBS, 0.05% BSA, 0.05% sodium azide. Also available without BSA and azide, see 168650-AF.
- Extra Details:
- It recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. This MAb is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise. Applications: Suitable for use in Immunofluorescence, Flow Cytometry, ELISA, Western Blot. Other applications not tested. Recommended Dilution: Flow Cytometry: 0.5-1ug/million cells Immunofluorescence: 0.5-1ug/ml Immunohistochemistry: Frozen & Formalin-fixed: 0.5-1ug/ml for 30 minutes at RT (No special pretreatment is required for the immunohistochemical staining of formalin-fixed, paraffin-embedded tissues. Optimal dilutions to be determined by the researcher. Positive Control: Biotinylated proteins in solution or on tissues Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice