168645-AF-PE
AMACR (Alpha-methylacyl-CoA Racemase, CBAS4, Da1-8, Macr1, RACE, RM, p504S) (PE)
Size
£876.00
- SKU:
- 168645-AF-PE
- Purity:
- Purified by affinity column.
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- A synthetic peptide from human AMACR protein. Cellular Localization: Cytoplasmic
- Format:
- Supplied as a liquid in 10mM PBS. No preservative added. BSA-Free. Labeled with R-Phycoerythrin (PE).
- Extra Details:
- This antibody recognizes a protein of 54kD, which is identified as AMACR, also known as p504S. It is an enzyme that is involved in bile acid biosynthesis and b-oxidation of branched-chain fatty acids. AMACR is expressed in cells of premalignant high-grade prostatic intraepithelial neoplasia (HGPIN) and prostate adenocarcinoma. The majority of the carcinoma cells show a distinct granular cytoplasmic staining reaction. AMACR is present at low or undetectable levels in glandular epithelial cells of normal prostate and benign prostatic hyperplasia. A spotty granular cytoplasmic staining is seen in a few cells of the benign glands. Applications: Suitable for use in Western Blot, Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:100-1:200 for 2 hours at RT Immunohistochemistry: formalin-fixed: 1:50-1:100 for 30-60 minutes at RT (Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA, pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 minutes Optimal dilutions to be determined by the researcher. Positive Control: HEK cells or Prostate Adenocarcinoma Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice