Phosphothreonine (HRP)

Size

£940.00

SKU:: 168433
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Phosphothreonine conjugated KLH
Format:: Supplied as a liquid in 0.02M potassium phosphate, 0.15M sodium chloride, pH 7.2. Labeled with horseradish peroxidase (HRP).
Extra Details:: Phospho Site Specific Phosphothreonine antibody is ideal for ELISA where Signal-to-noise ratio of phospho- versus nonphospho-peptides is critical. Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:1000 Western Blot: 1:500 Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.