168400
ORAI1 (ORAI1, Transmembrane Protein 142A, TMEM142A, Calcium Release-activated Calcium Channel Protein 1, CRACM1)
Size
£832.00
- SKU:
- 168400
- Purity:
- Purified by immunoaffinity chromatography from serum.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide corresponding to human ORAI1 near N-terminus.
- Format:
- Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide.
- Extra Details:
- Stimulation of immune cells triggers Ca++ entry through Ca++ release-activated Ca++ (CRAC) channels. Recently identified, ORAI1 is a four-transmembrane spanning protein that is an essential component of CRAC. A missense mutation in this protein in humans is the cause of one form of hereditary severe combined immune deficiency (SCID) which results in ablated T-cell Ca++ entry. It has been suggested that ORAI1 functions as a highly selective Ca++ plasma membrane channel that is gated through interactions with STIM1, the store-activated endoplasmic reticulum Ca++ sensor. Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:10,000-1:40,000 Western Blot: 0.5-1ug/ml, a ~51kD band is expected, often migrates at a higher than expected (~32kD) Immunohistochemistry: 10ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice