Myeloperoxidase (MPO, pANCA)

Size

£1,041.00

SKU:: 168381
Purity:: Purified by ion exchange chromatography
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Myeloperoxidase from human leukocytes
Format:: Supplied as a lyophilized powder from PBS, pH 7.2, 0.01% gentamicin sulfate. Reconstitute with 100ul sterile dH2O.
Extra Details:: Human myeloperoxidase (MPO) is a dimeric protein composed of two heavy subunits (53kD) and two light subunits 15kD. Each MPO molecule contains two prosthetic porphyrins which play an important role in the catalytic cycle. Molecular weights for MPO isoforms from pools of normal human samples range from 114,000 to 140,000 daltons reflecting a heterogeneous mixture of isoforms when assayed under non-reducing conditions of SDS-PAGE. Often MPO from a single donor will yield a homogenous preparation reflecting a single isoform. The carbohydrate component of MPO, consisting of mannose, glucose and N-acetylglucosamine residues is 2.5%. MPO is inhibited by azide and other compounds. MPO is stored in primary granules of neutrophils and serves as a bactericidal agent in that MPO catalyzes the production of hypochlorous acid (HOCl), a powerful oxidant. HOCl is derived from chloride ion (Cl-) and hydrogen peroxide (H2O2). In a number of inflammatory situations, MPO is released into the extracellular matrix where its measurement can be used as an indication of neutrophil activation. Applications: Suitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilutions: ELISA: 1:5000-1:20,000 Western Blot: 1:500-1:5000 Immunoprecipitation: 1:100 Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice