MIP-1B (CCL4, C-C motif chemokine 4, Small-inducible cytokine A4, Macrophage inflammatory protein 1-beta, MIP-1-beta, ACT-2, MIP-1b)

Size

£914.00

SKU:: 168363
Purity:: Purified by Protein A affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Recombinant protein corresponding to 69aa from porcine MIP-1B expressed in yeast. Species sequence homology: equine, panda (98%), bovine, canine, rabbit (97%), macaque, feline (95%), human, chimpanzee (94%), rat, and oppossum (91%).
Format:: Supplied as a lyophilized powder in PBS, pH 7.2, 0.01% sodium azide. Reconstitute with sterile dH2O or PBS.
Extra Details:: In many species, both C-C chemokines macrophage inflammatory protein, or MIP-1a (CCL3L1), and MIP-1b (CCL4) are structurally and functionally related CC chemokines. They are both potent chemoattractants for monocytes, which form an important component of the stroma of tumor tissue, and in humans may regulate tumor growth and associated inflammation. They participate in the host response to invading bacterial, viral, parasite and fungal pathogens by regulating the trafficking and activation state of selected subgroups of inflammatory cells e.g. macrophages, lymphocytes and NK cells. Both MIP-1a and MIP-1b exert similar effects on monocytes, but their effect on lymphocytes differ. MIP-1a selectively attracts CD8+ lymphocytes, while MIP-1b selectively attracts CD4+ lymphocytes. They contain the four highly conserved cysteine residues present in CC chemokines. MIP-1b(CCL4) has specificity for CCR5 receptors. In humans, it is also a major HIV-suppressive factor produced by CD8+ T cells. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 1:5000 Western Blot: 1:500 Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice