168350
MARK2, phosphorylated (T595) (ELKL motif kinase, EMK1, MAP/microtubule affinity regulating kinase 2, PAR1 homolog, Serine/threonine protein kinase MARK2)
Size
£914.00
- SKU:
- 168350
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide corresponding to rabbit MARK2, phosphorylated at T595.
- Format:
- Supplied as a liquid in 0.02M potassium phosphate, 0.15M sodium chloride, pH 7.2, 0.01% sodium azide.
- Extra Details:
- This recognizes a regulatory phosphorylation on a kinase that is critical to regulation of microtubules, cell cycle and immune responses. MARK2 refers to MAP/microtubule affinity- regulating kinase 2 isoform a [Homo sapiens]. EMK (ELKL Motif Kinase) is a small family of ser/thr protein kinases involved in the control of cell polarity, microtubule stability and cancer. Several cDNA clones have been isolated that encode two isoforms of the human ser/thr protein kinase EMK1 called MARK2. These isoforms were characterized by the presence of a 162bp alternative exon that gives rise to two forms, one containing the exon and the other one lacking it. Both forms were found to be co-expressed in a number of selected cell lines and tissue samples. Human MARK2 was shown to be encoded by a single mRNA that is ubiquitously expressed. This transcription variant includes the alternative exon in the coding region and therefore codes for a longer protein. Multiple splice variants exist for this protein. Applications: Suitable for use in Immunofluorescence, ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 1:1000-1:3000 Western Blot: 1:250-1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice