KLH (Keyhole Limpet Hemocyanin)

Size

£672.00

SKU:: 168322
Purity:: Purified by Protein A affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Native KLH, followed by hybridoma development
Clone:: 14B31
Format:: Supplied as a liquid in 0.02M potassium phosphate, 0.15M sodium chloride, pH 7.2, 0.01% sodium azide.
Extra Details:: KLH is used to detect Keyhole Limpet Hemocyanin. KLH is a copper-containing oxygen carrier occurring freely dissolved in the hemolymph of many mollusks and arthropods. KLH is often used in molecular immunology as a carrier protein conjugated to low molecular weight molecules such as peptides, aa’s, nucleic acids, drugs or toxins to render them more immunogenic due to the size of the conjugate complex and the immunogenicity of KLH. KLH forms a large complex composed of ~50kD subunits. Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:20,000-1:100,000 Western Blot: 1:2000-1:10,000 Immunohistochemistry: 1:1000-1:5000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.