Interleukin 2 (IL-2, IL2, Interleukin-2, T-cell Growth Factor, TCGF)

Size

£914.00

SKU:: 168311
Purity:: Purified by Protein A affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Recombinant protein corresponding to 134aa from feline IL-2 expressed in yeast..
Format:: Supplied as a lyophilized powder in 0.02M PBS, pH 7.2, 0.01% sodium azide. Reconstitute with sterile dH2O or PBS.
Extra Details:: IL-2 is a member of a family of cytokines, which also includes IL-4, IL-7, IL-9, IL-15 and IL-21. All members of this family signal through a receptor complex that includes the common G chain (?c). IL-2 signals through a receptor complex consisting of IL-2 specific IL-2 receptor A (CD25), IL-2 receptor B (CD122) and the common G chain (?c). The main function of IL-2 is to promote the development of T regulatory cells. IL-2 is produced by several cell types including, B cells, ?d T cells, CD4+ T cells, CD8+ T cells, and eosinophils. It has been found to be similar to IL-15 in terms of function. Both IL-2 and IL-15 are able to facilitate production of immunoglobulins made by B cells and induce the differentiation and proliferation of natural killer cells. The primary differences between IL-2 and IL-15 are found in adaptive immune responses: IL-2 participates in maintenance of T-Regs and reduces self-reactive T cells while IL-15 is necessary for maintaining highly specific T cell responses by supporting survival of CD8 memory T cells. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 1:20,000 Western Blot: 1:500-1:2000 Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile dH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice