His-Tag (6-Histidine, 6 His, 6-His Epitope Tag, HHHHHH Epitope Tag, HHHHHH Tag, Polyhistidine Tag) (HRP)

Size

£889.00

SKU:: 168242
Purity:: Purified by Protein A affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Synthetic peptide corresponding to 6X His epitope tag peptide H-H-H-H-H-H conjugated to KLH using maleimide.
Clone:: 33D10.D2
Format:: Supplied as a lyophilized powder in 0.02M potassium phosphate, 0.15M sodium chloride, pH 7.2, 0.01% gentamicin sulfate, 10mg/ml BSA. Reconstitute with sterile dH2O or PBS. Labeled with horseradish peroxidase (HRP).
Extra Details:: Epitope tags are short peptide sequences that are easily recognized by tag-specific. Due to their small size, epitope tags do not affect the tagged protein's biochemical properties. Most often, sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows epitope tags to serve as universal detection reagents for any tag-containing protein produced by recombinant means. This means that epitope tags are a useful alternative to generating specific to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The epitope tag is usually functional in a variety of dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells. Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:10,000 Western Blot: 1:1000-1:5000 Immunohistochemistry: 1:500-1:2500 Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder is stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O prior to use. To avoid repeated freezing and thawing, aliquot reconstituted stock solution and store at -20°C. Reconstituted product is stable for 12 months at -20°C. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice