168225
Glutathione S-Transferase (GST) (HRP)
Size
£869.00
- SKU:
- 168225
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Nartive full length GST isolated from Schistosoma japonicum.
- Format:
- Supplied as a lyophilized powder in 0.02M PBS, pH 7.2, 10mg/ml BSA. Reconstitute with sterile dH2O or PBS. Labeled with horseradish peroxidase (HRP). Do not add azide.
- Extra Details:
- GST (Glutathione-S-Transferase) is a protein expression tag commonly used in molecular biology. GST will react with synthetic construct present in most known GST containing cloning or expression vectors. GST is responsible for the conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. The aa sequence GST is highly conserved in most organisms including mammals. GST exists as a 26kD homodimer. Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:10,000-1:50,000 Western Blot: 1:1000-1:5000 Immunohistochemistry: 1:500-1:2500 Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile dH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light.
- Shipping Conditions:
- Blue Ice