168037
ASAP3 (DDEFL1, UPLC1, Arf-GAP with SH3 Domain, ANK Repeat and PH Domain-containing Protein 3, Development and Differentiation-enhancing Factor-like 1, Protein Up-regulated in Liver Cancer 1)
Size
£914.00
- SKU:
- 168037
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to human UPLC1/ASAP3.
- Format:
- Supplied as a liquid in 0.02M potassium phosphate, 0.15M sodium chloride, pH 7.2, 0.01% sodium azide.
- Extra Details:
- UPLC1 (up-regulated in liver cancer 1)/ASAP3, also named DDEFL1 (development and differentiation-enhancing factor-like 1) or ASAP3, is a member of the AZAP family of proteins. These proteins catalyze the hydrolysis of GTP bound to ADP-ribosylation factor (Arf) proteins, thereby causing Arf inactivation. For this reason, the ASAPs are generally called ArfGAPs. The activity of ArfGAPs is dependent on the presence of phosphoinositides and is implicated in cellular processes such as membrane trafficking and remodeling of the actin cytoskeleton. ASAP3 has been found to be up-regulated in 80% of the hepatocellular carcinomas examined. Initial biochemical characterization reveals that ASAP3 shows class-specific GAP activity on Arf proteins, preferring Arf5 over Arf1 and Arf6. ASAP3 has beed developed through the NCI collaboration program and is ideal for Cancer and Signal Transduction research. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: :1000-1:10,000 Western Blot: 1:200-1:2000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice