AKT (Protein Kinase B, PKB, Rac, Related to A and C kinases)

Size

£914.00

SKU:: 168014
Purity:: Affinity Purified
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Sheep
Immunogen:: Synthetic peptide corresponding to human AKT1.
Format:: Supplied as a liquid in 0.02M potassium phosphate, 0.15M sodium chloride, pH 7.2.
Extra Details:: AKT is a component of the PI-3 kinase pathway and is activated by phosphorylation at 473aa and 308aa. AKT is a cytoplasmic protein also known as Protein Kinase B (PKB) and rac (related to A and C kinases). AKT is a key regulator of many signal transduction pathways. AKT Exhibits tight control over cell proliferation and cell viability. Overexpression or inappropriate activation of AKT is noted in many types of cancer. AKT mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3-kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus. AKT has two main roles: (i) inhibition of apoptosis; (ii) promotion of proliferation. AKT is ideal for investigators involved in Cell Signaling, Neuroscience, Signal Transduction research. Applications: Suitable for use in Immunofluorescence, ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:5000-1:20,000 Western Blot: 1:500-1:2000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice