168005
ACTB (Actin, Cytoplasmic 1, Beta-actin)
Size
£793.00
- SKU:
- 168005
- Purity:
- Purified
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide corresponding to C-terminal region near aa350-375 of human beta Actin.
- Format:
- Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide.
- Extra Details:
- In vertebrates 3 main groups of actin isoforms, a, b and g have been identified. A-actins are found in muscle tissues and are a major constituent of the contractile apparatus. B and G-actins coexist in most cell types as components of the cyto-skeleton and as mediators of internal cell motility. B-actins are highly conserved proteins that are involved in cell motility, structure and integrity. B-actins are cytoplasmic proteins. Actin is a loading control and is critical for the correct interpretation of your western blot. B-Actin Loading Control is used to normalize the levels of protein detected by confirming that protein loading is uniform across the gel. Applications: Suitable for use in Immunofluorescence, Immunohistochemistry, ELISA and Western Blot. Other applications not tested. Recommended Dilution: Immunofluorescence: 1:500-1:2000 ELISA: 1:10,000-1:40,000 Western Blot: 1:1000-1:4000, band at ~42kD Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice