IL-1 beta, Recombinant, Human

Size

£524.00

SKU:: 167885
Molecular Weight:: 17.3
Purity:: ≥98% (HPLC, SDS-PAGE). Endotoxin: ≤0.1ng/ug
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a lyophilized powder. Reconstitute with dH2O to 0.1-1mg/ml following centrifugation.
Extra Details:: IL-1beta is a proinflammatory cytokine produced in a variety of cells, including monocytes, tissue macrophages, keratinocytes, and other epithelial cells. Both IL-1alpha and IL-1beta bind to the same receptor and have similar, if not identical, biological properties. These cytokines have a broad range of activities including the stimulation of thymocyte proliferation by inducing IL-2 release, B cell maturation and proliferation, mitogenic FGF-like activity, and the release of prostaglandin and collagenase from synovial cells. However, whereas IL-1beta is a secreted cytokine, IL-1alpha is predominantly a cell-associated cytokine. Recombinant human IL-1b is a 17.3kD protein containing 153aa residues. Recombinant protein corresponding to human IL-1b expressed in E.coli. Biological Activity: The ED50 as determined by the dose-dependent stimulation of thymidine uptake by murine D10S cells is ≤1pg/ml, corresponding to a specific activity of ≥1x10e9 units/mg. Amino Acid Sequence: APVRSLNCTL RDSQQKSLVM SGPYELKALH LQGQDMEQQV VFSMSFVQGE ESNDKIPVAL GLKEKNLYLS CVLKDDKPTL QLESVDPKNY PKKKMEKRFV FNKIEINNKL EFESAQFPNW YISTSQAENM PVFLGGTKGG QDITDFTMQF VSS Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice