167698
STAT3, phosphorylated (Y705) (Signal Transducer and Activator of Transcription 3)
Size
£850.00
- SKU:
- 167698
- Application:
- WB
- Purity:
- Purified by Protein G affinity chromatography from hybridoma culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rat
- Immunogen:
- Phosphopeptide containing the human STAT3 Y705 site
- Clone:
- 805435
- Format:
- Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Extra Details:
- Human STAT3/STAT3a (signal transducer and activator of transcription 3; also called APRF) is a 90-95kD member of the STAT family of transcription factors. It is 770aa in length, contains one SH2 domain (aa580-670), and is found in almost all cell types. STAT3 mediates gp130, LIFR, OBR, IL10R and EGFR signaling. Upon activation, receptors such as gp130 phosphorylate STAT3 at Ser727, which results in transcriptional activation. Phosphorylation at Tyr705 mediates homo and heterodimerization with STAT1 and nuclear translocation, and is an important event in oncogenic transformation. The STAT3b isoform shows a six aa substitution for aa716770, thus eliminating the Ser727 site. Human and mouse STAT3a share 99% aa sequence identity. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 2ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice