167695
Sirtuin 1 (Silent Mating Type Information Regulation 2 Homolog, SIRT1, SIR2alpha, SIR2L1, SIR2-like Protein 1) [KO Validated]
Size
£895.00
- SKU:
- 167695
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Sheep
- Immunogen:
- Recombinant protein corresponding to Ala2-Ser747 from human Sirtuin 1/SIRT1, expressed in E. coli.
- Format:
- Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 500ul sterile PBS.
- Extra Details:
- SIRT1 (SIR2like protein 1; also NADdependent protein deacetylase sirtuin1 and hSIR2) is a class I member of the sirtuin family of enzymes. Although its predicted MW is 81kD, it runs anomalously at 110120kD in SDSPAGE. It is a widely expressed nuclear protein that participates in the deacetylation of multiple proteins, including p300, p53, LKB1 and histone H1. Functionally, this has the effect of promoting heterochromatin formation, cell survival and resistance to oxidative stress. Metabolically, SIRT1 induces insulin secretion, inhibits glycolysis and suppresses fatty acid synthesis. Human SIRT1 is 747aa in length. It possesses two NLS's (aa3239 and 223230), an NES (aa138145), and a sertuintype deacetylase domain (aa241495) that contains an NAD and Zn binding motif. There are at least 12 utilized Ser/Thr phosphorylation sites, plus two nitrosylated Cys and one acetylated Ala. There are also four potential isoform variants. One is 95kD in size and shows a deletion of aa454639, a second is 17kD in size and contains a 16aa substitution for aa149747, and a third contains an alternative start site at Met296. SIRT1 is also known to undergo proteolysis by cathepsin B at Val533Ser534, generating a fourth, Cterminally truncated 75kD isoform. Fulllength SIRT1 is suggested to form trimers, while the 17kD isoform appears to form dimers. Over aa2747, human and mouse SIRT1 share 86%aa sequence identity. Applications: Suitable for use in Western Blot and Flow Cytometry. Other applications not tested. Recommended Dilution: Western Blot: 0.2ug/ml Flow Cytometry: 0.25ug/10e6 cells Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice